Table S2.

The affinity of the antibodies prepared via cytokine-assisted immunization

	KD(nM)	Kon(1/Ms)	kon Error	Koff(1/s)	Koff Error	Rmax	Rmax error	Requilibrium	X2	R2
6-20G5	0.14	2.08E+05	1.41E+03	2.93E-05	6.94E-06	2.592	0.005201	2.586	1.843	0.9985
6-8D2	2.09	4.35E+04	1.48E+02	9.08E-05	2.46E-06	1.685	0.004778	1.659	0.03397	0.9999
6-18H3	5.07	1.14E+05	4.07E+02	5.75E-05	4.70E-06	2.539	0.003137	2.529	0.8136	0.9994
6-5G8	0.7	9.57E+04	3.89E+02	6.67E-05	4.28E-06	2.607	0.00354	2.594	0.8124	0.9995
6-2A9	3.12	8.72E+04	3.62E+02	2.72E-04	5.17E-06	2.522	0.005042	2.465	0.7758	0.9994
6-3C10	19.34	6.42E+04	1.06E+03	1.24E-03	1.68E-05	2.504	0.03084	2.186	1.361	0.9977
6-18G10	0.31	1.92E+05	4.25E+02	5.93E-05	2.86E-06	2.413	0.002477	2.402	0.8382	0.9995
6-11C3	15.18	3.39E+05	6.16E+03	5.14E-03	6.85E-05	2.191	0.01945	1.784	6.697	0.9857

Note: The antibodies that K_D value were less than 5.3 nM could be regared as high-affinity antibodies.

Abbreviations: K_D, equilibrium dissociation constant; k_on, association rate constant; k_off, dissociation rate constant.