Figure 14.

Fit of complex concentration-response data with the present model, Case V: Activity and binding data for different opioids in cells expressing wildtype μ-opioid receptor in the cAMP (A) and β-arrestin2 (B) assays, respectively (after Hothersall et al., 2017) and fitted with the present model (Equation 4). Corresponding fractional response vs. occupancy plots generated using pK _i values derived from [³H]diprenorphine competition assays and fitted with the model (Equation 27) are shown for cAMP (C) and β-arrestin2 (D). A relative response plot (“bias plot”) showing the fractional responses plotted against each other is included in (E).