. 2019 Jan 15;316(5):E749–E772. doi: 10.1152/ajpendo.00343.2018

Table 4.

Estimated femoral material properties from WT and Nmp4^−/− mice treated with vehicle, raloxifene, parathyroid hormone, and parathyroid hormone + raloxifene

	Ultimate Stress	Yield Stress	Strain to Yield, µε	Total Strain, µε	Modulus, GPa	Resilience, MPa	Toughness, MPa
Group
WT VEH	141.12 ± 6.97	105.73 ± 12.89	16,012 ± 1124	99,029 ± 30,556	7.87 ± 0.30	0.95 ± 0.18	9.19 ± 1.93
Nmp4⁻^/⁻ VEH	150.08 ± 8.71	121.23 ± 21.47	16,982 ± 2858	82,378 ± 26,470	8.55 ± 0.54	1.08 ± 0.31	8.86 ± 2.59
WT RAL	155.49 ± 5.00	117.92 ± 5.13	16,552 ± 371	83,863 ± 19,659	8.06 ± 0.75	1.06 ± 0.12	9.15 ± 1.86
Nmp4⁻^/⁻ RAL	159.48 ± 13.63	127.00 ± 22.37	16,999 ± 2556	75,610 ± 19,939	8.24 ± 1.13	1.20 ± 0.35	8.95 ± 2.10
WT PTH	152.07 ± 4.20	97.09 ± 15.56	16,881 ± 3008	90,210 ± 29,366	7.57 ± 1.23	0.85 ± 0.28	9.46 ± 2.63
Nmp4⁻^/⁻ PTH	157.94 ± 5.37	97.77 ± 9.56	15,021 ± 1932	91,519 ± 26,401	7.93 ± 0.30	0.82 ± 0.23	10.31 ± 2.28
WT PTH + RAL	166.39 ± 3.78	102.76 ± 18.16	15,452 ± 34324	69,998 ± 19,460	8.44 ± 0.38	0.89 ± 0.32	8.16 ± 1.90
Nmp4⁻^/⁻ PTH + RAL	173.39 ± 10.84	114.52 ± 13.66	15,321 ± 708	63,635 ± 16,321	8.64 ± 1.06	0.99 ± 0.20	8.09 ± 1.91
Two-way ANOVA genotype	G: P = 0.0012	G: P = 0.0109	G: P = 0.7849	G: P = 0.1310	G: P = 0.0559	G: P = 0.1304	G: P = 0.8913
	Nmp4⁻^/⁻: 160.22^a	Nmp4⁻^/⁻: 115.13^a
	WT: 153.77^b	WT: 105.87^b
Two-way ANOVA treatment	T: P < 0.0001	T: P < 0.0001	T: P = 0.2740	T: P = 0.0016	T: P = 0.0318	T: P = 0.0031	T: P = 0.0553
	P + R: 169.89^a	R: 122.46^a		P: A 90864^a	P + R: 8.54^a	R: 1.13^a
	R: 157.48^b	V: 113.48^a,^b		V: A 90704^a	V: 8.21^a,^b	V: 1.01^a,^b
	P: 155.00^b	P + R: 108.64^b,^c		R: 79737^a,^b	R: 8.15^a,^b	P + R: 0.94^a,^b
	V: 145.60^c	P: 97.43^c		P + R: 66816^b	P: 7.55^b	P: 0.84^c
Two-way ANOVA G × T	G × T: P = 0.8141	G × T: P = 0.5067	G × T: P = 0.2220	G × T: P = 0.6425	G × T: P = 0.7598	G × T: P = 0.6545	G × T: P = 0.7901

Data represent average ± SD; n = 7–14 mice/group. Nmp4, nuclear matrix protein 4; WT, wild type; V/VEH, vehicle; R/RAL, raloxifene; P/PTH, parathyroid hormone; P/PTH + R/RAL, and parathyroid hormone + raloxifene; BV/TV, bone volume per total volume. Statistical analyses were performed using two-way ANOVA tests setting genotype (G) and treatment (T) as the independent variables. Statistical significance was set at P ≤ 0.05. The statistical results list the cohorts by genotype, treatment, and genotype × treatment.

^{a b c}Cohorts not connected by the same letter are statistically different; the average value of the specific parameter precedes the letter.