Fig. 4.
A–E: effect of insulin and exercise on pAktSer473/Akt in each fiber type [I (A), IIA (B), IIB (C), IIBX (D), and IIX (E)] from 3.5 h postexercise (3.5hPEX) and time-matched sedentary (SED) rats, incubated without or with a submaximally effective insulin dose. Open bar: no insulin from SED or 3.5hPEX group; filled bar: with insulin from SED or 3.5hPEX group. *Insulin versus basal (no insulin): type IIB, P < 0.001; IIBX, P < 0.05; IIX, P < 0.001 (SED group); and type I, P < 0.05; IIA, P < 0.01; IIB, P < 0.001; IIBX, P < 0.001; IIX, P < 0.05 (3.5hPEX group). ‡3.5hPEX versus SED with basal (no insulin): P < 0.05 in IIBX fibers. †3.5hPEX versus SED with insulin: P < 0.01 in type I and IIA fibers, P < 0.01 in IIX fibers. Data were analyzed by two-way ANOVA, and Tukey post hoc analysis was performed to identify the source of significant variance. Values are means ± SE; n = 3–6 per group. Representative blots and loading controls [myosin heavy chain (MHC)] are included for each fiber type.