Fig. 6.
A–E: effects of insulin and exercise on pAS160Ser704/AS160 in each fiber type [I (A), IIA (B), IIB (C), IIBX (D), and IIX (E)] from 3.5 h postexercise (3.5hPEX) and time-matched sedentary (SED) rats, incubated without or with a submaximally effective insulin dose. Open bar: no insulin from SED or 3.5hPEX group; filled bar: with insulin from SED or 3.5hPEX group. *Insulin versus basal (no insulin): type IIB, P < 0.05; IIX, P < 0.05 (SED group); and type I, P < 0.001; IIA, P < 0.05; IIB, P < 0.01; IIBX, P < 0.01; IIX, P < 0.05 (3.5hPEX group). ‡3.5hPEX versus SED with basal (no insulin); P < 0.05 in IIB fibers. †3.5hPEX versus SED with insulin: type I, P < 0.001; IIA fibers, P < 0.05; IIB, P < 0.05; IIBX, P < 0.01. Data were analyzed by two-way ANOVA, and Tukey post hoc analysis was performed to identify the source of significant variance. Values are means ± SE; n = 3–6 per group. Representative blots and loading controls [myosin heavy chain (MHC)] are included for each fiber type.