FIGURE 7.

Effect of MK801 on the [Ca²⁺]_i in stimulated MCs and TGs close to the stimulated MCs following mechanical stimulation of a single MC. (A) Traces of [Ca²⁺]_i increases in the stimulated MC (black trace) and its nearby TGs (red trace) in the presence of 10 μM MK801 (upper white box). (Responses from nearby TGs were recorded in cells at 11.0 μm from the stimulated MCs. The construction of horizontal dotted lines, upper filled boxes, and [Ca²⁺]_i increases through the application of high concentration of extracellular 100 mM K⁺ solution are the same as in Figure 4. (B) In the presence of 10 μM MK801, the F/F₀ value of the mechanically stimulated MCs (blue column) and nearby TGs (red columns); TGs located within 0–10.0 μm and 10.1–20.0 μm of the mechanically stimulated MCs are shown. Bars represent the mean ± SE. Numbers in parentheses indicate the tested cells. We could not observe any statistically significant differences between the F/F₀ values of the mechanically stimulated MCs in the absence (Figure 3B) and presence of 10 μM MK801 (P > 0.05). Statistically significant differences between the F/F₀ values of the TGs located at 0–10.0 μm and 10.1–20.0 μm from mechanically stimulated MCs in the absence (Figure 3B) and presence of MK801 (100 μM) ae observed. ^*P < 0.05.)