Fig. 1.

Immunolocalization of Bax and Bcl-2 (green) in control cells (placed on the frame of RPM). Cells were fixed and the two proteins were stained as described under Materials and Methods. Images of representative cells with Bax and Bcl-2 localization are shown. Bax and Bcl-2 were both mainly localized only in the cytoplasm for the whole duration of the experiment but, just as an example, the figure deals with Bax at 1 h (a) and Bcl-2 at 6 h (b), for which several nuclei positive for Bax and Bcl-2 signals can be appreciated. Nuclei are stained with propidium iodide (red). Scale bar = 10μm.