Fig. 5.

Evaluation of the Bax and Bcl-2 expression in nuclear fractions by WB analysis. Figure reports the WB analyses on isolated nuclei of C6 glioma cells. For each panels, the samples loaded were: lane 1, mitochondria enriched fraction (Mit), used as positive control; lane 2, nuclei from control C6 glioma cells (time 1 hour); lane 3, nuclei from control C6 glioma cells (time 6 hours); lane 4, nuclei from control C6 glioma cells (time 24 hours); lane 5, nuclei from C6 glioma cells treated with µg for 1 hour; lane 6, nuclei from C6 glioma cells treated with µg 6 hours; lane 7, nuclei from C6 glioma cells treated with µg for 24 hours. Panel A reports the whole protein pattern stained with Red Ponceau, full image in Supplementary section (Suppl6.F5a). Panels b and c show the semiquantitative Western Blot analysis of Bax, full image in Supplementary section (Suppl7.F5b) and Bcl-2, full image in Supplementary section (Suppl8.F5c). The ratios of these signals were analyzed by densitometry and normalized against the Red Ponceau signal (Panels d and e, respectively) The values of densitometric analysis are expressed as Relative Optical density (R.O.D.). Each panel is representative of at least five experiments and in Panels d and e data are expressed as mean ± S.D.