Table 1. Nucleotide sequences of mouse primers for RT-PCR.
Gene | GenBank No. | Primer sequence (5′-3′) |
β-actin | NM_007393.3 | F: GAT TAC TGC TCT GGC TCC TAG C |
R: GAC TCA TCG TAC TCC TGC TTG C | ||
LOX-1 | NM_138648.2 | F: AGG TCC TTG TCC ACA AGA CTG G |
R: ACG CCC CTG GTC TTA AAG AAT TG | ||
Dectin-1 | NM_020008.3 | F: GAC CCA AGC TAC TTC CTC |
R: GCA GCA CCT TTG TCA TAC T | ||
IL-1β | NM_008361.3 | F: CGC AGC AGC ACA TCA ACA AGA GC |
R: TGT CCT CAT CCT GGA AGG TCC ACG | ||
TNF-α | NM_013693.2 | F: ACC CTC ACA CTC AGA TCA TCT T |
R: GGT TGT CTT TGA GAT CCA TGC | ||
MIP-2 | NM_009140.2 | F: TGT CAA TGC CTG AAG ACC CTG CC |
R: AAC TTT TTG ACC GCC CTT GAG AGT GG | ||
IL-10 | NM_010548.2 | F: TGC TAA CCG ACT CCT TAA TGC AGG AC |
R: CCT TGA TTT CTG GGC CAT GCT TCT C |
RT-PCR: Reverse transcriptase polymerase chain reaction; IL: Interleukin; TNF: Tumor necrosis factor; MIP: Macrophage inflammatory protein.