| Sample details |
| Organism |
E. coli
|
| Source |
E. coli expressed |
| NCBI reference sequence ID |
NC_000913.3 (1–186) |
| Extinction coefficient [A
280, 0.1%(w/v)] |
1.006 |
| Partial specific volume (cm3 g−1) |
0.74 |
| Partial contrast from sequence and solvent constituents, ρprotein − ρsolvent (1010 cm−2) |
2.79 (12.28–9.48) |
| Data-collection parameters |
| Beam size at sample (µm) |
266 × 266 |
| Wavelength (Å) |
1.2398 |
|
q-range (Å−1) |
0.0013–0.48 |
| Detector |
Rayonix MX170HS and LX170HS |
| Absolute scaling method |
Comparison with Milli-Q water scattering in the same cell |
| Normalization |
To transmitted intensity by beam-stop counter |
| Monitoring for radiation damage |
X-ray dose maintained below 210 Gy, frame-by-frame comparison |
| Detector distance (m) |
8.5 and 1.0 |
| Exposure time per image (s) |
8 |
| Sample volume (µl) |
250 |
| Sample configuration |
1.4 mm path-length capillary-flow cell at 4 µl s−1
|
| Data-collection temperature (K) |
295 |
| Structural parameters from Guinier plot |
|
I(0) (cm−1) |
0.12 |
0.14 |
0.15 |
0.15 |
|
R
g (Å) |
42.1 |
43.1 |
43.2 |
42.0 |
|
q
min (Å−1) |
0.0014 |
0.0014 |
0.0014 |
0.0014 |
|
qR
g max (q
min = 0.0082 Å) |
1.3 |
1.3 |
1.3 |
1.3 |
| Structural parameters from P(r) plot |
|
I(0) (cm−1) |
0.118 |
0.142 |
0.150 |
0.155 |
|
R
g (Å) |
42.7 |
42.9 |
42.7 |
42.1 |
|
D
max (Å) |
115.7 |
131.5 |
126.6 |
120.8 |
| Porod volume estimate (nm3) |
293 |
339 |
359 |
371 |
| Volume of correlation (Å3) |
345 |
1090 |
2070 |
2410 |
| Molecular-mass determination |
| Molecular mass M
r [V
c
2/(0.1231R
g)] (kDa) |
253 |
283 |
290 |
300 |
| Calculated monomeric M
r from sequence (kDa) |
21.9 |
