Table 2. Data-collection and refinement statistics for the single-particle reconstruction of TMV.
| Data collection | |
| Accelerating voltage (kV) | 300 |
| Nominal magnification | 130 000 |
| Calibrated pixel size (Å) | 1.067 |
| Total No. of movies collected | 357 |
| Total fluence (e− Å−2) | 40 |
| No. of frames | 40 |
| Defocus range (µm) | −1.0 to −3.0 |
| Data processing | |
| Final segments | 109763 |
| Helical parameters | |
| Twist (°) | 22.04 |
| Rise (Å) | 1.41 |
| Gold-standard resolution (Å) | 2.3 |
| MolProbity statistics | |
| All-atom clashscore | 2.41 |
| Ramachandran plot | |
| Outliers (%) | 0 |
| Allowed (%) | 3.97 |
| Favoured (%) | 96.03 |
| Rotamer outliers (%) | 0 |
| Cβ deviations (%) | 0 |
| Deviations from ideal values | |
| Bond lengths (µm) | 0.007 |
| Bond angles (°) | 1.030 |
| Chirality (Å3) | 0.060 |
| Planarity (°) | 0.009 |
| Dihedral (°) | 8.534 |
| Map–model CC | |
| Main chain (760 atoms) | 0.68 |
| Side chain (513 atoms) | 0.63 |
| EMDB ID | EMD-4928 |