Fig. 11.
Proliferation of primary human proximal tubules. Cells were transfected with empty vector control, histone deacetylase-1 (HDAC1), or HDAC4 plasmids 24 h before incubation with vehicle (0.1% DMSO) or the pan-HDAC inhibitor, trichostatin A (TSA), and the 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt proliferation reagent for 24 h. %Change from vector control is reported ±SE. n = 5 independent cell experiments for vehicle groups, n = 3 independent cell experiments for TSA groups. Two-factor ANOVA, *P < 0.05 compared with vector vehicle group. +P < 0.05 compared with HDAC4 vehicle group from Tukey post hoc analysis. PTxT, PTransfection×Treatment.