Fig. 1.

Blood glucose level (A), body weight (B), urine output (C), and ratio of kidney weight to body weight (D) in male wild-type (WT-M), female wild-type (WT-F), male endothelial nitric oxide synthase-deficient (eNOS^−/−) db/db (Db-M), and female eNOS^−/− db/db (Db-F) mice. In A–C, *P < 0.05 (one-way ANOVA), Db-M vs. WT-M and Db-F vs. WT-F mice; †P < 0.05, WT-M vs. WT-F mice. In A, 5–7 mice were used in all age groups of WT-M and WT-F mice. In B and C, 8–10 mice were used in all age groups of WT-M and WT-F mice. However, for eNOS^−/− db/db mice in A–C, 9–13 mice were used in the age groups of 4–20 wk and 5 mice in the group of 24 wk for both Db-M and Db-F mice. In D, ratios of kidney weight (KW) to body weight (BW) were evaluated at 24 wk. **P < 0.01 (Student’s t-test), comparison between the groups indicated. n, number of mice in each group.