Fig. 5.

Abundance of renal cortical fibronectin (FN) and collagen IV (Col IV) proteins in male and female wild-type (WT) and endothelial nitric oxide synthase-deficient (eNOS^−/−) db/db mice. A and C: representative Western blots of renal cortical extracts showing the protein abundance of FN (A) and Col IV (C) in the cortex of kidney from WT male (WT-M) and female (WT-F) mice and eNOS^−/− db/db male (Db-M) and female (Db-F) mice at 24 wk. β-Actin was used as the loading control. B and D: summary densitometric data from experiments presented in A and C, respectively. *P < 0.05 and **P < 0.01 (Student’s t-test), comparison between the groups as indicated. n, number of mice in each group.