Fig. 6.

Glomerular expression of fibronectin (FN) and collagen IV (Col IV) in male and female wild-type (WT) and endothelial nitric oxide synthase-deficient (eNOS^−/−) db/db mice at an age of 24 wk. A and C: representative light microscopic images of immunohistochemistry of FN (A) and Col IV (C). Original magnification was ×200. B and D: semiquantitation of glomerular stain of FN (B) and Col IV (D) from four male and five female WT mice (WT-M and WT-F, respectively) and seven male and female eNOS^−/− db/db mice (Db-M and Db-F, respectively). About 15–20 glomeruli from 1 kidney section were counted, and 5 sections were taken from 1 mouse. **P < 0.01 (Student’s t-test), comparison between the groups as indicated.