Fig. 5.

Short-time apoptotic response of proximal tubule cells (PTCs) coincubated with high glucose and albumin. A: cartoon illustrating the uptake of high glucose (red arrow) and albumin (purple arrow) in PTCs. B−D: quantification of the apoptotic index (B), Bax abundance (C), and Bax accumulation on mitochondria (D) in PTCs incubated with control (5.6 mM), 10 mM glucose (HG10)-, 2.5 mg/ml albumin-, or 10 mM glucose + 2.5 mg/ml albumin-containing medium for 8 h. Data are expressed as means ± SE; n = 9 coverslips from 3 individual cell preparations. *P < 0.05; **P < 0.01; ***P < 0.001.