Table 1.
Ouabain rescues PTCs and MCs from high glucose-induced apoptosis
| Variable | Cell Type | Control | Glucose | Glucose + Ouabain | Number of Coverslips (Number of Preparations) |
|---|---|---|---|---|---|
| AI, % | PTC | 3.1 ± 0.1 | 7.5 ± 0.3 | 2.9 ± 0.2 | 9 (3) |
| Bcl-xL expression, % | PTC | 100 | 68 ± 4 | 97 ± 7 | 15 (5) |
| Bax expression, % | PTC | 100 | 155 ± 14 | 124 ± 9 | 15 (5) |
| Bax/mitochondrial overlap, % | PTC | 100 | 144 ± 14 | 118 ± 9 | 15 (5) |
| Δψm, % | PTC | 100 | 66 ± 6 | 89 ± 5 | 3 (3) |
| ROS, % | PTC | 100 | 143 ± 9 | 118 ± 11 | 8 (2) |
| AI, % | MC | 3.1 ± 0.3 | 5.3 ± 0.7 | 3.3 ± 0.5 | 12 (4) |
| Bcl-xL expression, % | MC | 100 | 62 ± 3 | 97 ± 6 | 12 (4) |
| Bax expression, % | MC | 100 | 162 ± 19 | 118 ± 19 | 13 (5) |
| Bax/mitochondrial overlap, % | MC | 100 | 153 ± 24 | 96 ± 14 | 13 (5) |
Values are means ± SE. Shown is the quantification of the apoptotic index (AI), Bcl-xL abundance, Bax abundance and accumulation on mitochondria, mitochondrial membrane potential (Δψm), and ROS in proximal tubule cells (PTCs) and mesangial cells (MCs) incubated with control (5.6 mM), 15 mM glucose-, or 15 mM glucose + 5 nM ouabain-containing medium for 8 h.