Table 2.

Ouabain rescues PTCs from high glucose- and albumin-induced apoptosis

Variable	Cell Type	Control	Glucose + Albumin	Glucose + Albumin + Ouabain
Apoptotic index, %	PTC	3.0 ± 0.2	7.4 ± 0.4	3.4 ± 0.4
Bax expression, %	PTC	100	149 ± 12	104 ± 8
Bax/mitochondrial overlap, %	PTC	100	170 ± 22	124 ± 16

Values are means ± SE; n = 9 coverslips from 3 individual cell preparations. Shows is the quantification of the apoptotic index, Bax abundance, and accumulation of Bax on mitochondria in proximal tubule cells (PTCs) incubated with control (5.6 mM), 15 mM glucose + 2.5 mg/ml albumin-, or 15 mM glucose + 2.5 mg/ml albumin + 5 nM ouabain-containing medium for 8 h.