Fig. 6.
Genetic knockdown of Period 1 (Per1) reduces epithelial Na+ channel (ENaC) activity at the single channel level in Xenopus 2F3 distal nephron cells. 2F3 cells were transfected with fluorescein-labeled siRNA directed against Per1 (siPer1) or scrambled control (Con). ENaC activity was assessed by a cell-attached single channel patch-clamp technique. A: number of channels [channel density (N)] times the open probability (Po) of the channel (NPo). B: N. C: Po. The number of recordings made is indicated above each bar. D: representative tracing from a control siRNA-transfected cell compared with a siPer1-transfected cell. C, closed; O, open. Data are presented as means ± SE. Data were analyzed by an unpaired t-test. *P < 0.05; ***P < 0.01.