Table 1.
Primers used for real-time PCR
| Primer | Sequence |
|---|---|
| Tlr3-forward | 5′-CCAGAAGAATCTAATCAAATTAGATTTGTC-3′ |
| Tlr3-reverse | 5′-TTTTGCTAAGAGCAGTTCTTGGAG-3′ |
| Tlr4-forward | 5′-GGCAACTTGGACCTGAGGAG-3′ |
| Tlr4-reverse | 5′-CATGGGCTCTCGGTCCATAG-3′ |
| AT1R-forward | 5′-GCTGGCATTTTGTCTGGATAAC-3′ |
| AT1R -reverse | 5′-GCTTTTCTGGGTTGAGTTGG-3′ |
| Mmp9-forward | 5′-GAAGGCAAACCCTGTGTGTT-3′ |
| Mmp9-reverse | 5′-AGAGTACTGCTTGCCCAGGA-3′ |
| Nox4-forward | 5′-CTCTACTGGATGACTGGAAACC-3′ |
| Nox4-reverse | 5′-AGTCAGGTCTGTTTTCTTGCC-3′ |
| Nppa-forward | 5′-GTCTTGGCCTTTTGGCTTC-3′ |
| Nppa-reverse | 5′-TTCCTCAGTCTGCTCACTC-3′ |
| Tnf-forward | 5′-TGCCTATGTCTCAGCCTCTTC-3′ |
| Tnf-reverse | 5′-GAGGCCATTTGGGAACTTCT-3′ |
| Tgfb2-forward | 5′-GCTAATGTTGTTGCCCTCCT-3′ |
| Tgfb2-reverse | 5′-GCAGCAATTATCCTGCACATT-3′ |
| Gapdh-forward | 5′-CATTTCCTGGTATGACAATGAATACG-3′ |
| Gapdh-reverse | 5′-TCCAGGGTTTCTTACTCCTTGGA-3′ |
AT1R, angiotensin II receptor type 1; Mmp9, matrix metalloprotease 9; Nppa, atrial natriuretic peptide; Nox4, NADPH-oxidase 4; Tgfb2, transforming growth factor beta 2; Tlr3, Toll-like receptor 3; Tlr4, Toll-like receptor 4; Tnf, tumor necrosis factor-alpha.