Asymmetric charge partitioning factor of a) 10+ to 15+ streptavidin (SA), b) 11+ to 17+ transthyretin (TTR), c) 12+ to 17+ hemoglobin (Hb), and d) 17+ to 19+ and 23+ to 27+ C-reactive protein (CRP) upon activation with HCD and low- and high-energy UVPD (1 pulse). The ACPF for Hb is shown as the average for α- and β-subunits. The lab frame collision energy was optimized for each precursor and is equal to 1000 eV for SA (a), 1200 eV for TTR and Hb (b and c), and 2000 eV for CRP (d). The ACPF for purely symmetric charge partitioning (1.0) is shown in each graph. Error bars are equal to standard deviation of replicate data.