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. 2019 Jun 17;19:599. doi: 10.1186/s12885-019-5808-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — SZ168 detects hPDPN expression in the lung micro-metastasis foci and subcutaneous tumors in IHC analysis. The subcutaneous tumors (a) and lungs (d, g) were stained with H&E. Scale bar, 20 μm. Arrows indicate cancer nest. The subcutaneous tumors and lungs were incubated with SZ168 (b, e, h) or 18H5 (c, f, i) and then by the Envision⁺ kit reagents. Color was developed using DAB and subsequently counterstained with hematoxylin. Scale bar, 200 or 20 μm. Arrows indicate cancer nest