Fig. 3.

Phylogram of amino acid sequences of SpmHTs and other BAHD proteins. a, Phylogenetic tree constructed with SpmHTs and other BAHD proteins. Eggplant SHTs and SpmHTs are marked by solid blue and purple dots, respectively. The phylogram was generated by the neighbor-joining method following alignment by the ClustalW algorithm using Geneious software (version 4.8.5). The values represented the genetic distance under the Jukes-Cantor model. Arabidopsis lyrata AlHCT (EFH70827), Arabidopsis thaliana AtHCT (AT5G48930), AtSCT (AT2G25150), AtSDT (AT2G23510), AtSHT (AT2G19070), Capsicum annuum CaSHT1 (Capana00g001430), CaSHT2 (Capana05g000927), CaSpmHT (CA09g07820), Coffea canephora CcHCT (EF137954), CcHQT (EF153931), Cynara cardunculus CyHCT (DQ104740), CyHQT1 (EU697935), CyHQT2 (EU839580), Lycopersicon esculentum LsHQT (AJ582652), Nicotiana attenuata NaDH29 (JN390824), NaCV86 (JN390825), NaAT1 (JN390826), Nicotiana tabacum NtSHT (unannotated), NtHQT (AJ582651), NtHCT (CAD47830), Sorghum bicolor SbHCT (XP_002452435), Solanum lycopersicum SlSHT (Solyc07g015960.1.1), SlSpmHT (Solyc12g010980.1.1), Solanum melongena SmSHT (KP165410), SmSpmHT (KP233218), Solanum richardii SrSHT (KP165411), SrSpmHT (KR150683), Solanum tuberosum StSHT (PGSC0003DMP400059459), StSpmHT (Sotub12g006790.1.1), StSpmHTL (Sotub12g006750.1.1), Trifolium pratense TpHCT (EU861218), Vitis vinifera VvHCT (XP_002268988), and VvSHT (XP_002269790). b, Alignment of conserved regions of eggplant SpmHT and other polyamine hydroxycinnamoyl transferases. The conserved motifs ‘HXXXD’ and ‘DFGWG’ are marked by an orange and purple box, respectively