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(A) Sequences of previously reported peptides 1–218 and newly synthesized peptides 4a, 4b, 5, and 6. The numbers above each amino acid indicate the position in the prodomain sequence of mouse myostatin. (B–D) Luciferase reporter assay experiments determined the activities of peptides 4a, 4b, 5, and 6 toward myostatin inhibition. Peptide concentration: 10 (B,C) and 3 μM (D). Results are presented as mean values ± SD (n = 3).
