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. 2019 Jun 17;20:223. doi: 10.1186/s12882-019-1394-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Effect of administration of adnisodamine/atropine on cleaved caspase-3 in rats subjected to glycerol-induced AKI. a Cleaved caspase-3 immunostaining in cross sections of rat kidney at 24 h in untreated, glycerol-treated, and adnisodamine/atropine intervention after glycerol treatment groups over the time course. Cleaved caspase-3 positive staining was observed on the proximal tubular epithelial cells and damaged tubules. U, untreated; G, glycerol treatment alone group; GAP, glycerol atropine treatment group. GAD, glycerol adnisodamine treatment group. Bars = 50 μm. Data are the mean ± SEM from three separate experiments. b. The statistic evaluation of immunostaining of Cleaved caspase-3 (*P < 0.05 versus G24 group). c. Total kidney tissue extracts were analyzed for cleaved caspase-3 protein levels by western blot. Data are expressed as mean ± S.E. (n = 3), statistically significant from respective 0 h controls. *, statistically significant from rats receiving glycerol treatment alone at the corresponding time point (p < 0.05)