Table 3.
QTLs identified for three seed-germination related traits (GR, SL & FW) in LM6 and ZM6 RIL populations under natural aging treatment
| Aging treatment | Trait | QTL | Chromosome (Linkage group) | Position (cM) | Marker | LOD value | Additive effect | R2 value (%) |
|---|---|---|---|---|---|---|---|---|
| Natural-LM6 | GR | qGR-5-1 | 5 (A1) | 52.31 | bin502-bin503 | 3.63 | −0.05 | 11.13 |
| qGR-5-2 | 5 (A1) | 60.91 | bin512-bin513 | 5.07 | −0.05 | 15.05 | ||
| qGR-17–1 | 17 (D2) | 50.41 | bin1872-bin1873 | 3.49 | −0.06 | 12.98 | ||
| SL | qSL-17–1 | 17 (D2) | 38.21 | bin1864-bin1865 | 4.77 | −1.04 | 14.53 | |
| qSL-17-2 | 17 (D2) | 45.41 | bin1872-bin1873 | 4.44 | −1.04 | 15.08 | ||
| FW | qFW-4-1 | 4 (C1) | 92.61 | bin426-bin427 | 3.70 | 2.18 | 10.80 | |
| qFW-5-1 | 5 (A1) | 60.91 | bin512-bin513 | 4.46 | −2.45 | 13.56 | ||
| qFW-11-1 | 11 (B1) | 76.41 | bin1201-bin1202 | 3.00 | 2.03 | 8.97 | ||
| Natural-ZM6 | GR | qGR-5-3 | 5 (A1) | 71.91 | bin571-bin572 | 6.64 | 0.06 | 16.76 |
| qGR-7-1 | 7 (M) | 114.81 | bin891-bin892 | 3.68 | 0.05 | 9.43 | ||
| qGR-15–1 | 15 (E) | 104.01 | bin1942-bin1943 | 3.78 | −0.05 | 9.13 | ||
| SL | qSL-6-1 | 6 (C2) | 82.61 | bin681-bin682 | 3.20 | − 0.83 | 8.33 | |
| qSL-8-1 | 8 (A2) | 70.51 | bin958-bin959 | 3.04 | −0.80 | 7.82 | ||
| qSL-18–1 | 18 (G) | 96.51 | bin2290-bin2290 | 3.19 | −0.81 | 8.16 | ||
| FW | qFW-5-2 | 5 (A1) | 71.91 | bin571-bin572 | 3.25 | 1.87 | 8.57 | |
| qFW-9-1 | 9 (K) | 36.01 | bin1118-bin1119 | 5.43 | 2.44 | 14.89 |
GR (germination rate), SL (seedling length) and FW (fresh weight); R2 = Coefficient of determination/phenotypic variance explained (PVE). In natural aging condition, control was not used, and we used treatment trait value for QTL analysis