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Binding of Cltx-derived peptides to NRP1. Cltx-derived peptides were generated through trypsin digest of full length native Cltx or by peptide synthesis. NRP1 binding was assessed by BLI; biotinylated peptides were attached to biosensors and evaluated for binding to NRP1 (0 to 1750 nM). Binding affinity data (KD) represents mean of 2 to 6 independent experiments. ns = no significant binding
