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. 2019 Jun 17;7:93. doi: 10.1186/s40168-019-0711-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Response of microbial communities to HHQ exposure. Difference in growth rate (d⁻¹) in heterotrophic prokaryote abundance after 24 h exposure to 410 μM (100 ng mL⁻¹) 2-heptyl-4-quinolone (HHQ) compared to the DMSO control determined by flow cytometry over eight experimental time points (a). Bars represent the mean (± s. d.) of biological triplicate and asterisks indicate significant changes in the growth rate of the bulk communities between the two treatments (p < 0.05). Ordination of heterotrophic prokaryote communities based on particle-associated 16S rRNA (b) and free-living 16S rRNA (c) amplicon sequence variants visualized by non-metric multidimensional scaling (NMDS) of Bray-Curtis distance. Circles and triangles correspond to DMSO control and HHQ exposed communities, respectively. Symbol color denotes experimental time point.