FIG 3.

Enriched genes are involved in autophagy pathways. (A) Gene ontology (GO) enrichment on 273 significantly enriched genes revealed enrichment of 13 GO terms. The red line indicates an adjusted P value of 0.05. (B) Directed acyclic graph of relationships between the 13 GO terms identified in panel A and the 18 of 273 genes annotated to these processes. Two additional GO terms, “autophagy of nucleus” and “autophagy,” were propagated from their child terms. (C) Twenty-five genes were targeted by at least two enriched sgRNAs. Black bars represent genes carried forward for validation. Genes labeled “A” were involved in the GO autophagy annotations in panel B.