TABLE 1.
Gene ontology (GO) terms associated with enriched genes that were targeted by at least two sgRNA species
| Function(s) | GO term | Genesa |
|---|---|---|
| Autophagy | Late nucleophagy | ATG9A, ATG7 |
| Piecemeal microautophagy of the nucleus | RB1CC1, ATG7 | |
| Autophagy | ATG10, RB1CC1, ATG7 | |
| Autophagy of mitochondrion | RB1CC1, ATG12, ATG9A, ATG3, ATG7 | |
| Autophagosome assembly | RB1CC1, TMEM41B, ATG12, ATG9A, ATG3, ATG7 | |
| Macroautophagy | VPS37A, ATG10, RB1CC1, ATG12, ATG3, ATG7 | |
| Stress response | Cellular response to oxidative stress | TXNRD1, NFE2L2 |
| Cell redox homeostasis | TXNRD1, NFE2L2 | |
| Proteostasis and protein modification | Cellular protein modification process | ATG3, ATG7 |
| Proteasome-mediated ubiquitin-dependent protein catabolic process | NFE2L2, CUL3 | |
| Positive regulation of protein modification process | ATG10, ATG7 | |
| Protein modification by small-protein conjugation | ATG10, ATG7 | |
| Proteasomal ubiquitin-independent protein catabolic process | KEAP1, NFE2L2 | |
| Posttranslational protein modification | KEAP1, CAND1, CUL3 | |
| Protein ubiquitination | KEAP1, NFE2L2, ATG3, ATG7, CAND1, CUL3 | |
| Trafficking | Protein targeting to membrane | VPS37A, ATG3 |
| Transmembrane transport | SLC39A9, LRRC8A | |
| Intra-Golgi vesicle-mediated transport | RAB6A, GOSR1 | |
| Retrograde transport, endosome to Golgi apparatus | RAB6A, GOSR1 | |
| ER-to-Golgi vesicle-mediated transport | GOSR1, CUL3 | |
| Protein lipidation | ATG10, ATG7 | |
| C-terminal protein lipidation | ATG12, ATG7 | |
| Protein transport | ATG10, ATG9A, ATG7, GOSR1 | |
| Growth and development | Aging | NFE2L2, ATG7 |
| Immune system | Viral process | KEAP1, NFE2L2, RAB6A |
| Neutrophil degranulation | ATG7, RAB6A, CAND1, TUBB |
a
All genes were found in the blasticidin-selected samples and are statistically significantly enriched. TXNRD1, a bona fide NRF2 target gene, is in bold.