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Microinjection of MPF plus CKS2 or CKS1 alone rescues the GVBD delay in cks2−/− oocytes. Oocytes were injected with the indicated proteins. and the timing of GVBD was assessed by microscopic examination. Data represent the mean ± SD. Significance was determined by Student's t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
