Table 1.

PYY(1-36), (P³L³¹P³⁴)PYY(1-36), and PYY(Lys¹²PAL) amino acid sequences and characterisation.

Peptide name	Amino acid sequence	Purity (%)	Expected mass (Da)	Calculated mass (Da)
PYY(1-36)	YPIKPEAPGEDASPEELNRYYASLRHYLNLVTRQRY	99.9	4309.8	4309.7
(P3L31P34)PYY(1-36)	YPPKPEAPGEDASPEELNRYYASLRHYLNLLTRPRY	99.2	4277.9	4274.7
PYY(1-36)(Lys12PAL)	YPIKPEAPGEDK(PAL)SPEELNRYYASLRHYLNLVTRQRY	100	4606.4	4606.4

Abbreviations: MALDI-TOF MS, matrix-assisted laser desorption ionisation time-of-flight mass spectrometry; PYY, peptide tyrosine tyrosine; RP-HPLC, reverse phase high-performance liquid chromatography.

Peptides’ purity was confirmed using RP-HPLC on a ThermoQuest SpectraSYSTEM UV2000 chromatography system using a Phenomenex C-18 analytical column with absorbance at 214 nm. Identity of peptides was confirmed using Voyager-DE Biospectrometry MALDI-TOF MS (PerSeptive Biosystems, Framingham, MA, USA), as described previously.³²