Figure 3.

Secreted βig-h3 was quantified using ELISA in the culture supernatants. The results shown are representative of two independent experiments that included three different CAF preparations and two different ductal preparat. Secreted βig-h3 modulates specific CD8⁺ T cell responses. (A) Pancreatic draining lymph nodes were obtained from KC mice and cultured with mitomycin-treated KC cell line in the presence anti-βig-h3 Ab or ctrl Ab for 5 days. Representative dot plots show the CFSE dilutions of CD8⁺ T cells expressing CD44. The graph indicates the % of CFSE^lowCD8⁺CD44^high T cells. Student’s t-test *p<0.05 (B) Representative dot plots show the expression of CD44 and PD-1 in CD8⁺ T cell population. The graph indicates the % of CD44⁺PD-1⁺ among CD8+ T cells. ANOVA (F=13.49, ***p<0.001) and Tukey’s post hoc test. Anti-βig-h3-treated group compared with ctrl Ab group (**p<0.01) and to the non-stimulated group (***p<0.01) (C) Representative dot plots show the expression of Tim-3 and CD8 among CD8⁺CD44⁺PD-1⁺ cells. The graph indicates the % of Tim-3⁺ among CD8+CD44+PD-1+ T cells. ANOVA (F= 44.08, ***p<0.001) and Tukey’s post hoc test. Anti-βig-h3 treated group compared with ctrl Ab group (***p<0.01) and the non-stimulated group compared with ctrl Ab group (***p<0.01). The results shown are representative of three independent experiments. Ab, antibody; ANOVA, analysis of variance; CFSE, 5,6-carboxyfluorescein diacetate succinimidyl ester; KC, p48-Cre; Kras^G12D; Tim-3, T cell immunoglobulin and mucin-domain containing-3.