TABLE 2.

Primer/probe sequences used for qRT-PCR

Target and primer or probe name	Primer sequence	Nucleotide positiona
DNA Pol		65880–65953
Forward	5′-AGA GGG ACA TCC AGG ACT TTG T-3′
Reverse	5′-CAG GCG CTT GTT GGT GTA C-3′
Probe	5′-ACC GCC GAA CTG AGC A-3′
VP16		103946–104191
Forward	5′-CCT CGA TGG TAG ACC CGT AA-3′
Reverse	5′-ACA TTC GCG AGC ACC TTA AC-3′
Probe	5′-CAT AAA GTA CCC AGA GGC-3′
ICP27		113945–114034
Forward	5′-GCC CGT CTC GTC CAG AAG-3′
Reverse	5′-GCG CTG GTT GAG GAT CGT T-3′
Probe	5′-CAG CAC CCA GAC GCC-3′
LAT Intron		119721–119795
Forward	5′-ACC CAC GTA CTC CAA GAA GGC-3′
Reverse	5′-TAA GAC CCA AGC ATA GAG AGC CA-3′
Probe	5′-TCC CAC CCC GCC TGT GTT TTT-3′
ICP0		121385–121453
Forward	5′-GGC CGA GGG AGG TTT CC-3′
Reverse	5′-CCG CTT CCG CCT C-3′
Probe	5′-CTC CCA GGG CAC CGA C-3′
ICP4		130224–130292
Forward	5′-CAC GGG CCG CTT CAC-3′
Reverse	5′-GCG ATA GCG CGC GTA GA-3′
Probe	5′-CGA CGC GAC CTC C-3′

^aPosition according to GenBank accession no. NC_001806.