TABLE 1.
siRNA sequences against COX-2, EP2, and EP4
| Gene and target | Oligonucleotide sequencea |
|---|---|
| COX-2(PTGER2) | |
| siRNA 1 | 5′-ACCTCGATTGACAGCCCACCAACATATCAAGAGTATGTTGGTGGGCTGTCAATCTT-3′ |
| 5′-CAAAAAGATTGACAGCCCACCAACATACTCTTGATATGTTGGTGGGCTGTCAATCG-3′ | |
| Scrambled 1 | 5′-ACCTCGTGCCCAACACGCAATTAACATCAAGAGTGTTAATTGCGTGTTGGGCACTT-3′ |
| 5′-CAAAAAGTGCCCAACACGCAATTAACACTCTTGATGTTAATTGCGTGTTGGGCACG-3′ | |
| siRNA 2 | 5′-ACCTCGTGGGATGATGAGCAGCTATTTCAAGAGAATAGCTGCTCATCATCCCACTT-3′ |
| 5′-CAAAAAGTGGGATGATGAGCAGCTATTCTCTTGAAATAGCTGCTCATCATCCCACG-3′ | |
| Scrambled 2 | 5′-ACCTCGCTGTGAGTGCGAAGAATTGTTCAAGAGACAATTCTTCGCACTCACAGCTT-3′ |
| 5′-CAAAAAGCTGTGAGTGCGAAGAATTGTCTCTTGAACAATTCTTCGCACTCACAGCG-3′ | |
| EP2 (PTGER2) | |
| siRNA 1 | 5′-ACCTCGCGCCTACGTCAACAAGTTCATCAAGAGTGAACTTGTTGACGTAGGCGCTT-3′ |
| 5′-CAAAAAGCGCCTACGTCAACAAGTTCACTCTTGATGAACTTGTTGACGTAGGCGCG-3′ | |
| Scrambled 1 | 5′-ACCTCGGCCGAACTAAGCCCACTTTATCAAGAGTAAAGTGGGCTTAGTTCGGCCTT-3′ |
| 5′-CAAAAAGGCCGAACTAAGCCCACTTTACTCTTGATAAAGTGGGCTTAGTTCGGCCG-3′ | |
| EP4 (PTGER4) | |
| siRNA 1 | 5′-ACCTCGCTCCTTCATCCTCCTCTTCTTCAAGAGAGAAGAGGAGGATGAAGGAGCTT-3′ |
| 5′-CAAAAAGCTCCTTCATCCTCCTCTTCTCTCTTGAAGAAGAGGAGGATGAAGGAGCG-3′ | |
| Scrambled 1 | 5′-ACCTCGCTCCTCTCCCCTTCTCTATTTCAAGAGAATAGAGAAGGGGAGAGGAGCTT-3′ |
| 5′-CAAAAAGCTCCTCTCCCCTTCTCTATTCTCTTGAAATAGAGAAGGGGAGAGGAGCG-3′ |
a
The boldface at 5' ends represents sequences for duplex stability, which provide resistance to RNase degradation. Boldface in the middle of a segment represents the site for hairpin loop folding. Boldface at the 3' region represent oligonucleotide overhangs, which are resistant to nuclease degradation.