FIG 6.

Analysis of ICP0 protein expression during explant-induced reactivation. Thin sections were cut from formalin-fixed, paraffin-embedded TG sections from latently infected mice and TG explants incubated in MEM plus 2% stripped FBS (with or without DEX) for 8 or 16 h. IHC was performed using an anti-ICP0 antibody (Santa Cruz Biotechnology; sc-53070) that was diluted 1:50 and then biotinylated goat anti-mouse IgG (Vector Laboratories). The arrows indicate TG neurons recognized by the ICP0 antibody. The blue arrows indicate TG neurons with ICP0 primarily in the cytoplasm. Magnification, ×400 for all sections except 16 h plus DEX, which was ×600.