FIG 1.

Description of the viral and cell passages performed with HAstV MLB1 and MLB2 on selected cell lines. (A) Passage history in Huh-7.5 cells. Initially, cells were infected using clinical stool samples as an inoculum. After 7 to 8 viral passages of HAstV MLB1 and MLB2, respectively, infected cells were subcultured. (B) Huh-7AI and A549 cell passages using Huh-7.5 cell lysates as an inoculum to establish persistent infections. V-Pn, viral passages; C-Pn, cellular passages; EM, electron microscopy; IF, immunofluorescence; IFN, interferon.