FIG 5.

Viral genome titers detected in the culture supernatant (SN) by RT-qPCR assays of MLB1 and MLB2 strains in persistently infected cell lines. (A) Blue line refers to the Huh-7.5 cell line, red lines refer to the Huh-7AI cell line, green lines refer to the A549 cell line, and the orange line refers to the Caco-2 cell line. Continuous lines with squares refer to HAstV MLB1 strain, and dotted lines with triangles refer to HAstV MLB2 strain. The dotted blue line with squares corresponds to the MLB1 persistently infected Huh-7.5 cell line, using the original stool sample as an inoculum. (B) Mean viral genome titers of HAstV MLB1 and MLB2 detected by RT-qPCR assays in the SN of persistently infected Huh-7AI and A549 cell lines. The average was calculated based on 7 to 12 numbers, corresponding to the viral genome titers at each cell passage. Error bars indicate one standard deviation. The mean viral titers were significantly different between HAstV MLB1 and HAstV MLB2 in a comparison of the same infected cell line (*, P = 0.0009 in Huh-7AI cells, P = 0.0018 in A549 cells, Mann-Whitney test).