FIG 8.

IFN-β and IFN-λ1 expression in infected cell lines. (A) Temporal analysis of IFN-β and IFN-λ1 mRNA expression during acute infection in Huh-7 and A549 cells. Cells were infected with an MOI of 1,000 and 20 genome copies/cell for MLB1 and MLB2, respectively, and poly(I·C)-transfected cells were used as controls. (B and C) Immunofluorescence images correspond to an acute infection using the highest MOI possible (25,000 genome copies/cell for MLB1 and 680 genome copies/cell for MLB2). (D) Analyses of IFN-β and IFN-λ1 expression in persistently infected cultures. Mock, noninfected cells; Mock-PolyIC, noninfected cells transfected with poly(I·C) (positive control); persistent, persistently infected cells; persistent-PolyIC, persistently infected cells that were additionally transfected with poly(I·C); 1d, 2d, 4d, and 7d are the days postinfection (dpi) where IFN expression was measured during acute infections. All samples were quantified at least in duplicate from two distinct experiments.