TABLE 5.
Flow cytometry antibodies used in this study
| Antibody | Vendor | Catalog no. | Clone | Fluorochromea |
|---|---|---|---|---|
| Ki-67 | BD Biosciences | 556026 | B56 | FITC |
| CD45RA | BD Biosciences | 555488 | HI100 | FITC |
| HLA-DR | BD Biosciences | 555811 | G46-6 | FITC |
| CD57 | BD Biosciences | 555619 | NK-1 | FITC |
| CD45RA | BD Biosciences | 555489 | HI100 | PE |
| CD28 | BD Biosciences | 348047 | L293 | PE |
| CD107a | BD Biosciences | 562628 | H4A3 | PE-CF594 |
| CCR7 (CD197) | BD Biosciences | 562381 | 150503 | PE CF594 |
| CD4 | BD Biosciences | 341654 | SK3 (Leu3a) | PerCP-Cy5.5 |
| CD4 | BD Biosciences | 562659 | RPA-T4 | BV605 |
| CD27 | BD Biosciences | 337169 | L128 | APC |
| CD45RA | BD Biosciences | 560673 | HI100 | Alexa Fuor 700 |
| CD3 | BD Biosciences | 641397 | SK7 | APC-H7 |
| CD25 | BD Biosciences | 560225 | M-A251 | APC-H7 |
| CD8 | BD Biosciences | 560347 | RPA-T8 | V450 |
| CD8 | BD Biosciences | 560774 | RPA-T8 | V500 |
| FoxP3 | BD Biosciences | 560047 | 259D/C7 | Alexa Fuor 488 |
| CD95 | Biolegend | 305612 | DX2 | APC |
| CD38 | Biolegend | 303532 | HIT2 | BV605 |
| PD-1 (CD279) | Biolegend | 329924 | EH12.2H7 | BV605 |
| FoxP3 | eBiosciences | 12-4777-42 | 236A/E7 | PE |
| CD3 | eBiosciences | 45-0036-42 | SK7 | PerCP-Cy5.5 |
| LIVE/DEAD fixable dye | Life Technologies | L34957 | NA | AmCyam/V500 |
a
FITC, fluorescein isothiocyanate; PE, phycoerythrin; PerCP, peridinin chlorophyll protein; APC, allophycocyanin.