TABLE 1.
Ultrastructural quantification of secondary envelopment of HCMV virus particles in vACs of infected HFFs at 120 h postinfection
| Virus | No. of cells analyzed | No. of capsids total | % particles by type (mean ± SD)a
|
||
|---|---|---|---|---|---|
| Enveloped | Budding | Naked | |||
| TB40-BAC4 | 30 | 1,040 | 83.9 ± 14.8 | 15.5 ± 14.7 | 0.5 ± 1.7 |
| TB71del327-361 | 22 | 211 | 31.5 ± 18.9 | 65.5 ± 20.3 | 2.9 ± 5.3 |
| TB71del348-361 | 25 | 980 | 26.4 ± 12.4 | 72.1 ± 10.9 | 1.5 ± 2.8 |
| TB71mutK348-351A | 15 | 514 | 21.9 ± 14.0 | 76.5 ± 14.3 | 1.7 ± 3.1 |
| TB71revK348-351A | 14 | 418 | 77.1 ± 10.7 | 20.5 ± 6.8 | 2.4 ± 5.4 |
| TB71mutL357-358A | 17 | 599 | 82.5 ± 8.8 | 13.8 ± 8.5 | 3.7 ± 3.2 |
| TB71stop | 30 | 1,564 | 25.9 ± 8.6 | 69.9 ± 9.7 | 3.5 ± 4.3 |
a
Given are the mean percentages and standard deviations (SD) of enveloped particles, budding particles (nonenveloped particles attached to membranes), and naked particles (nonenveloped particles not attached to membranes) from at least two independent experiments. For each virus, boldface value highlights the stage of secondary envelopment at which the majority of particles were counted.