FIG 8.

Duration of reactivation and level of latency in ocularly infected mice. For analysis of explant reactivation in infected mice, WT and type 1 ILC-, type 2 ILC-, and type 3 ILC-deficient mice were ocularly infected as described in the legend to Fig. 6. On day 28 p.i., TG from infected mice were harvested for explant reactivation. Each individual TG was incubated in 1.5 ml of tissue culture medium at 37°C, and the presence of infectious virus was monitored for 15 days. Reactivation is based on 20, 7, 17, and 22 TG for WT and type 1 ILC-, type 2 ILC-, and type 3 ILC-deficient mice, respectively. The average time that the TG from each group first showed CPE ± SEM is shown. The P value was determined using one-way ANOVA. For analysis of the levels of latency in the TG of latently infected mice, WT and type 1 ILC-, type 2 ILC-, and type 3 ILC-deficient mice were ocularly infected as described in the legend to Fig. 6. On day 28 p.i., TG were harvested from the latently infected mice. Quantitative RT-PCR was performed on the individual TG from each mouse. GAPDH expression was used to normalize the relative expression of LAT RNA in the TG. LAT copy numbers per TG were measured using pGEM5317, a LAT-containing plasmid, as we described previously (74). Latency was based on 20, 10, 20, and 18 TG for WT and type 1 ILC-, type 2 ILC-, and type 3 ILC-deficient mice, respectively. The P value was determined using one-way ANOVA. (A) Time of reactivation in TG of latently infected mice. (B) LAT expression in TG of latently infected mice.