FIG 1.
Increased MeV replication in OBC in the absence of IFNAR signaling. (A) MeV IC323-EGFP dissemination in hippocampus slices from SLAM tg mice infected on the day of culture with 10,000 PFU, followed by fluorescence imaging over 72 h. Pictures were reconstituted using the MosaicJ plug-in with ImageJ software. (B) Amounts of viral nucleoprotein RNA copies accumulated over 3 days in hippocampus slices (n = 5) from SLAM and SLAM × IFNARKO mice infected with 10,000 PFU of MeV at day 0, 3, and 7 of ex vivo explant culture. MeV N RNA was quantified by RT-qPCR and normalized to the standard deviation for GAPDH mRNA. Statistical analyses were performed using the Mann-Whitney U test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.