FIG 3.

Schematic representation of Nipah virus and Hendra virus G and F chimeras. (A) G chimeras were constructed by exchanging the head domain between the two viral G glycoproteins. HeV G with an NiV G head domain (HG₁₆₇NG) and NiV G with an HeV G head domain (NG₁₆₇HG) were constructed by overlapping PCR mutagenesis. (B) F chimeras were constructed by exchanging the transmembrane domain (TM) and the cytoplasmic tail (CT) between NiV and HeV F glycoproteins. The TM and CT domains in F are strongly conserved between the two viruses and have previously been implicated in viral budding (44, 45). HeV F with an NiV F TM/CT domain (HF₄₈₈NF) and NiV F with an HeV F TM/CT domain (NF₄₈₈HF) were constructed by overlapping PCR mutagenesis. Protein modifications, including N-glycosylation (in G, G1 to G7; in F, G1 to G5) and O-glycosylation (o) sites, as well as the cathepsin L cleavage site in F (Cat L), are depicted in G (A) and/or F (B).