FIG 10.

Characterization of FeLV TP2R. (A) Viral copies and titers of FeLV-A 61E and FeLV TP2R harvested from the supernatants of FeLV-infected AH927 cells are shown as copies per milliliter and the 50% tissue culture infectious doses (TCID₅₀) per milliliter. (B) FeLV proteins were detected in AH927 cells infected with FeLV-A 61E (61E) or FeLV TP2R (TP2R) using anti-FeLV gp70 Env and anti-FeLV p27 Gag antibodies by Western blot analysis. (C) Interference assay of FeLV TP2R. Env-pseudotyped viruses of FeLV-A (FeLV-A clone 33), FeLV-B (FeLV-B Gardner-Arnstein), FeLV-C (FeLV-C Sarma), FeLV-D (FeLV-D Ty26), and FeLV TG35-2 were tested for infection of AH927 cells and AH927 cells preinfected with FeLV TP2R (AH927/TP2R). (D) The replication-competent viruses of FeLV-A 61E, FeLV TP2R, and ampho-MuLV carrying LacZ were tested for infection of MDTF, MDTF-feTHTR1, and MDTF-feRFC cells. The infection units are by log₁₀ of β-galactosidase (LacZ)-positive cells per milliliter of supernatant. Data represent the averages from three independent experiments, with the standard deviations shown.