FeLV replication in different cell lines. The cells (CRFK, Fet-J, MCC, 3201, and MS4) were infected with 2 × 103 TCID50 of FeLV TP2R or FeLV-A 61E virus. The viral copy number was measured in the culture supernatants at 10 days postinfection by quantitative real-time RT-PCR. The y axis indicates the viral copy number. **, P < 0.01, *, P < 0.05 (Student’s t test). Data represent the averages from three independent experiments, with the standard deviations shown.