FIG 3.

Infection of cell lines by LacZ-carrying Env-pseudotyped viruses. (A) The expression of RFC was tested in the cell lines HeLa, HeLa-R5 (R5), R5 expressing human RFC (R5-huRFC), R5 expressing feline RFC (R5-feRFC), and R5 expressing mouse RFC (R5-mRFC) by RT-PCR. β-Actin was used as a control. (B) The Env-pseudotyped viruses of FeLV-A (FeLV-A clone 33), FeLV-B (FeLV-B Gardner-Arnstein), FeLV-C (FeLV-C Sarma), FeLV-D (FeLV-D Ty26), and FeLV TG35-2 were tested for infection in the cell lines shown on the x axis. (C) The Env-pseudotyped viruses of FeLV TG35-2 were tested in the cell line R5 expressing mouse RFC (R5-mRFC), shown on the x axis. The y axis indicates the infection units for log₁₀ of β-galactosidase (LacZ)-positive cells per milliliter of supernatant. Data represent the averages from three independent experiments, with the standard deviations shown.