RFC expression in feline tissues and feline cell lines. Detection of RFC by RT-PCR using total RNA isolated from the indicated tissues and cell lines (AH927, CRFK, Fet-J, MCC, 3201, and MS4). A representative 2% agarose gel with electrophoresed PCR product (305 bp) is shown. The gels were stained by ethidium bromide. RT(+) and RT(−) controls were included during cDNA synthesis.