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ICP0 HTS assay. (A) GST-ICP0 fusion proteins used for the HTS assay. ICP0 exon II spans amino acids 20 to 241 of ICP0 from HSV-1(F), while the portion of exon III of ICP0 that was used as a control spans amino acids 543 to 768 (ICP0 exon III-C). (B and C) HTS assay measuring the proximity of the ubiquitin (Ub) moiety to ICP0.
