FIG 6.

Effects of selected hits on HSV-1 and ΔICP0 virus gene expression. (A) U2OS cells were infected with HSV-1(F) or ΔICP0 virus (0.1 PFU/cell) and either not treated or treated with compound A, C, G, or H at 10 or 100 μM, added to the cultures at the time of infection. The cells were harvested at 2, 4, and 8 h postinfection, and viral gene expression was assessed by qPCR analysis. The 18S rRNA primers were used for normalization. (B) HEp-2 cells were treated as in Fig. 4 and pictures were acquired at 24 h postinfection, using an inverted Nikon Eclipse TE2000-S microscope equipped with a Nikon camera.